Entrapment of calf-thymus DNA on magnetic nanoparticles

Sameena Y; Israel V. M. V. Enoch

doi:10.34256/famr2015

Sameena Y Department of Chemistry, Sri Shakthi Institute of Engineering and Technology, Coimbatore – 641062, Tamil Nadu, India.
Israel V. M. V. Enoch Department of Chemistry, Department of Nanoscience, Karunya Institute of Technology and Sciences, Coimbatore - 641114, Tamil Nadu, India.

DOI: https://doi.org/10.34256/famr2015

Keywords: Magnetic nanoparticles, Glycol chitosan, DNA, Entapment

Abstract

Magnetic nanoparticles can be used to load bio–molecules for various biophysical applications. The direct attachment of bio–molecules such as protein and DNA to magnetic nanoparticles may lead to alter their structure. A method to immobilize and store biomolecules for example DNA on to a magnetic nanoparticles surface without much structural alteration is carried out in the present work. DNA is a target for many therapeutic small molecules as therapeutic drugs bind to DNA – interfering with protein factors involved in DNA mechanism, or cleave DNA cross-link – interfering with the cell division. This may find applications in drug interactions study with the stored DNA without much alteration in their structure.

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